(4) Enzymes
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Enzymes are protein catalysts involved in the regulation of…
biochemical reactions in metabolic pathways.
Enzymes increase…
the rate of reaction without being altered or consumed during a reaction.
Enzymes ___ the activation energy of a reaction.
Lower.
Role of oxidoreductases.
Catalyze oxidation-reduction reactions.
Role of transferases.
Catalyze group transfer of C, N, or P containing groups.
Role of hydrolases.
Catalyze cleavage bonds by the addition of water.
Role of lyases.
Catalyze cleavage of C-C, C-S, & certain C-N bonds, lysis generating a double bond.
Role of isomerases.
Catalyze optical or geometric isomers, rearrangements.
Role of ligases.
Catalyze the formation of bonds between C, O, S, N; coupled of hydrolysis of high energy phosphates (ATP required).
Reference range of AST.
5 - 30 U/L.
Reference range of ALT.
6 - 37 U/L.
Reference range of CPK.
15 - 160 U/L (slightly lower in females).
Reference range of GGT.
6 - 45 U/L (slightly lower for females).
Reference range of LDH.
Forward: 100-225 U/L. Reverse: 80 - 290 U/L.
Reference range of ALP.
30 - 90 U/L.
Reference range of AMY.
95 - 290 U/L.
State the enzyme class for each of the following analytes: AST, ALT, CK, GGT
Transferases.
State the enzyme class for each of the following analytes: LDH, G-6-PD
Oxidoreductase.
State the enzyme class for each of the following analytes: PChE, AMY, LIP, ALP, ACP
Hydrolases
State the enzyme class for each of the following analytes: ALD
Lyase.
State the optimal pH for the following analyte: Amylase
6.9 - 7.0
State the optimal pH for the following analyte: ALP
10
State the optimal pH for the following analyte: ACP
5.0
State the optimal pH for the following analyte: Lipase
8.6 - 9.0