REAGENT PREPARATION AND LABORATORY SUPPLIES

Specifications established by the American Chemical Society (ACS); Labels on reagents state the actual impurities for each chemical lot OR list the maximum allowable impurities; Suitable for use in most analytic laboratory procedures

Additional purification steps; for use in specific procedures such as chromatography, atomic absorption, immunoassays, molecular diagnostics, standardization or other techniques that require extremely pure chemicals

Impurity limitations are not stated; preparation of these chemicals is not uniform; melting point analysis is often used to ascertain the acceptability purity range; not recommended for use unless further purification or a reagent blank is included

Used to manufacture drugs; limitations are based on the criterion of not being injurious to individuals; purity standards are not based on laboratory needs

used primarily in manufacturing and should never be used in the clinical laboratory

Glass or plastic utensils used to transfer liquids

Holds or contains a particular volume but does not dispense that exact volume

will dispense the volume indicated

continuous etched ring or two small, close continuous rings located near the top of the pipet

Allows the contents of the pipet to drain by gravity

What drainage characteristics does serologic pipets have?

What drainage characteristics does Mohr pipet have?

Dispense or transfer aqueous solutions; self-draining

For biologic fluids having a viscosity greater than that of water, blow out

Most routinely used pipet

Types of Automatic pipets:

Piston-operated devices. A disposable, one time use polypropylene tip is attached to the pipet barrel. The pipet is placed into the liquid to be aspirated and is drawn into a dispensed from this tip.

Use a capillary tip that may be siliconized glass, glass, or plastic. This type of pipet is useful if a reagent reacts to plastics.

Allows the tubes to attain a horizontal position when spinning and a vertical position when the head is not moving; can attain speeds of up to approximately 3000 rpm

have angled compartments for the tubes and allow small particles to sediment more rapidly

The angle of the angle-head centrifuge is fixed at around:

high-speed centrifuges used to separate layers of different specific gravities; they are commonly used to separate lipoproteins (used in the reference methods for lipoprotein quantitation)

Centrifuge care: - Calibration is done very: - Disinfect on a: - Check speed using: