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State 5 points that sum up PCR
• PCR is a technique by which any sequence of DNA can be cloned or amplified • There are no need for cloning vectors, or transformation of bacterial cells followed by screening programme • DNA is amplified entirely in vitro, in an automated system involving a thermocycler • PCR is rapid and selective that can produce billions of coies of DNA sequences in a few hours. • This is much faster than the time taken for transformation of bacteria.
What process can be used to analyse DNA and PCR products?
Gel Electrophoresis
How can gene technology be used?
• Medicine - pharmaceutical products and diagnosis of disease • Gene therapy • Animal cloning • Transgenic animals/plants • DNA sequencing • Pharmacogenetics • DNA fingerprinting
What is DNA fingerprinting?
Uses DNA to be able to distinguish between and/or individuals
When would DNA fingerprinting be used?
• Paternity cases • Victim identification • Identifying suspects
When was Dolly born?
1996
Dolly was the first what?
The first mammal to be cloned from an adult somatic cell
Who was Dolly cloned by?
Scientists at Roslin Institute, Edinburgh
Where was the donor cell taken from?
Taken from white sheep
Who was the surrogate mother?
Black faced sheep
What is a good example of a GM crop?
Golden Rice
What does it contain?
Beta-carotene