producing DNA fragments using the gene machine
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define biosafety
aims to protect public health and environment from accidental exposure to biological agents
define biosecurity
deals with the prevention of miuse through loss,theft,diversion, intentional release of pathogens
what are the advantages of using a gene machine
+ quick + no introns (prokaryotes cannot splice) + any sequence of nucleotides + accurate
what are the 3 ways to isolate gene fragments
• reverse transcriptase • restriction endonucleases • gene machine
explain how gene machine is used to produce DNA fragments
• desired protein • amino acid sequence • mRNA sequence • DNA sequence • nucleotide base sequence into a computer -biosafety/biosecurity/international standards/ethics check • computer designs oligonucleotides (small, overlapping, single stranded nucleotides) • oligonucleotides assembled one nucleotide at a time (automated) • oligonucleotides joined to form gene (all coding DNA. single strand) • PCR replicates gene (makes complementary strand for DNA first) • gene into plasmid using sticky ends (vector for storage/cloning/transfer) • genes check using sequencing techniques