ALT, AST, ALP LEC
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old name of ast
serum glutamicoxaloacetic transaminase
what class does ast belong
transferase
coenzyme of ast
pyridoxal phosphate
what cycle in ast provide the source of energy
tricarboxylic acid cycle
ast has the highest concentration in what tissue source/s
Cardiac tissue Liver Skeletal muscle
in cytoplasm (got 1) it is mainly from?
RBC and heart
in got 1 the predominant form occurring is in
serum
in mitochondria (got 2) it is present in
liver
T/F: Isoenzyme analysis is routinely performed
F
clinical use of ast
Evaluation of hepatocellular disorders and skeletal muscle involvement
how many ULN in viral hepatitis
100x ULN
how many ULN in cirrhosis
4x ULN
what is the indicator reaction in karmen method principle
malate dehydrogenase
ph of ast
7.3-7.8
what will happen if there is hemolysis in ast
dramatically increases serum AST concentration
old name of alt
serum glutamicpyruvic transaminase
coenzyme of alt
pyridoxal phosphate
where can you find alt in the tisue sources?
liver
Confined mainly to evaluation of hepatic disorders
alt
ratio of ast and alt if there is acute hepatocellular injury
7,000:3,000 ratio
what is the indicator enzyme of wrobleski ladue reaction
lactate dehydrogenase
absorbance of ast and alt
340nm
T/F: alt is unaffected by hemolysis
T
name of alkaline phosphatase
Monoester Phosphohydrolase (Alkaline Optimum)