Midterm Lec 1
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Energy travels as electromagnetic waves, which have two key features:
Wavelength and frequency
→ distance between two wave peaks -Measured in NANOMETERS (nm)
Wavelength
the wavelength (in nm) where light passes through the most (peak transmittance)
Nominal wavelength
_ errors in setting the wavelength can cause big mistakes in absorbance readings
small
Energy (E) and wavelength are connected by
Planck’s formula
Planck’s formula:
Shorter wavelength → higher energy
Wavelength Regions Visible spectrum:
400 - 700 nm
Wavelength Regions Ultraviolet region (UV):
<400 nm
Wavelength Regions Infrared region (IR):
> 700 nm
Wavelength Regions Planck’s formula:
E = hv
Planck’s formula: E means
is the energy of a photon in Joules
Planck’s formula: h means
constant (6.626 x 10-27 erg sec) → Bishop
Planck’s formula: v means
frequency
the number of vibrations of wave motion per second.
Frequency
The lower the wave frequency, the longer the _
wavelength
The wavelength is inversely related to _ and _; the _ the wavelength, the higher the frequency and energy, and vice versa.
frequency and energy shorter
how many wave vibrations happen each second →Measured in _
Frequency Hz (Hertz)
Low frequency =
longer wavelength
High frequency =
shorter wavelength & more energy
Colorimetry the measurement of light intensity in a narrower wavelength.
Spectrophotometric measurement
Colorimetry the measurement of light intensity using a specific wavelength
Photometric measurement
It involves measurement of the light transmitted by a solution to determine the concentration of the light-absorbing substances in the solution.
Spectrophotometry
a device that measures the wavelengths of light or the intensity of radiation.
Spectrophotometry
It means =
Transmitted light thru the sample