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A - CHAPTER III: PHLEBOTOMY

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

– collection of minute sample of blood through capillary puncture

Back (Answer)

MICROSAMPLING

#2
Front (Question)

INFANTS up to 1 year of age

Back (Answer)

• Plantar surface of the big toe • Median or lateral side of the heel • Depth of puncture: up to 2.4 mm

#3
Front (Question)

ADULTS:

Back (Answer)

• Middle or ring finger • Puncture must be slightly off-center, perpendicular to the fingerprint • Margin of the earlobe • Depth of puncture: up to 3.1 mm

#4
Front (Question)

– collection of a greater volume of blood from the veins or arteries

Back (Answer)

MACROSAMPLING

#5
Front (Question)

ARTERIAL PUNCTURE:

Back (Answer)

• Collection of arterial blood for blood gas analysis or blood pH determination • Common sites: radial artery, brachial artery, femoral artery

#6
Front (Question)

• Collection of venous blood

Back (Answer)

VENIPUNCTURE

#7
Front (Question)

Infants up to 18 months:

Back (Answer)

External jugular vein Superior longitudinal sinus Temporal vein

#8
Front (Question)

18 months to 3 years:

Back (Answer)

Popliteal vein Femoral vein Long saphenous vein Ankle vein

#9
Front (Question)

3 years to adulthood:

Back (Answer)

Veins on the antecubital fossa Wrist vein Veins on the dorsal of hands & feet

#10
Front (Question)

3 years to adulthood:

Back (Answer)

Veins on the antecubital fossa Wrist vein Veins on the dorsal of hands & feet

#11
Front (Question)

METHODS OF PERFORMING VENIPUNCTURE:

Back (Answer)

I. OPEN SYSTEM II. CLOSED SYSTEM

#12
Front (Question)

– use of syringes

Back (Answer)

OPEN SYSTEM

#13
Front (Question)

• The hub of the needle is color-coded, corresponding to its gauge

Back (Answer)

OPEN SYSTEM

#14
Front (Question)

• The lower the gauge, the bigger the bore of the needle

Back (Answer)

OPEN SYSTEM

#15
Front (Question)

– use of an evacuated system (evacuated tube, two-way needle and adapter)

Back (Answer)

CLOSED SYSTEM

#16
Front (Question)

• Evacuated tubes are equipped with a hemogard (color-coded in accordance to the additive present)

Back (Answer)

CLOSED SYSTEM

#17
Front (Question)

• Multiple sampling can be carried out

Back (Answer)

CLOSED SYSTEM

#18
Front (Question)

– liquid portion of clotted blood

Back (Answer)

SERUM

#19
Front (Question)

– liquid portion of anti-coagulated blood

Back (Answer)

PLASMA

#20
Front (Question)

: preparation involves the removal of proteins from any biological specimen to prevent direct colorimetric interference by the formation of zwitterions at isoelectric pH where proteins exhibit maximum precipitation and minimum solubility

Back (Answer)

PROTEIN-FREE FILTRATE (PFF)

#21
Front (Question)

METHODS of PFF PREPARATION:

Back (Answer)

A. Physical Methods: 1. Heat 2. Ultracentrifugation B. Chemical Methods (ACID Method) 1. Folin-Wu 2. Hayden’s method 3. Van Slyke 4. TCA (5%) (BASE Method) 1. Nelson-Somogyi

#22
Front (Question)

▪ Specimen: whole blood; plasma/serum ▪ 2/3 N Sulfuric acid ▪ 10% sodium tungstate

Back (Answer)

Folin-Wu

#23
Front (Question)

▪ Specimen: serum ▪ N/12 Sulfuric acid ▪ 10% sodium tungstate

Back (Answer)

Hayden’s method

#24
Front (Question)

• makes use of a pre-mixed acid

Back (Answer)

Van Slyke