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17. Chromatography

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Total cards: 23•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (23)

#1
Front (Question)

GC - order of elution and resolution is highly dependent on..?

Back (Answer)

Stationary phase

#2
Front (Question)

Elution method where the mobile phase remains constant throughout the experiment

Back (Answer)

Isocratic elution

#3
Front (Question)

How is quantitative analysis performed in chromatography?

Back (Answer)

Measured parameter = peak area

#4
Front (Question)

Most common form of HPLC used in the clinical lab?

Back (Answer)

Reversed phase partition HPLC

#5
Front (Question)

Organic compound typically used in a polar mobile phase?

Back (Answer)

Acetonitrile Methanol

#6
Front (Question)

Normally, the ______ phase is nonpolar, and the ________ phase is polar

Back (Answer)

Mobile = nonpolar Stationary = polar

#7
Front (Question)

HPLC - separation depends on these 2 variables

Back (Answer)

• Solute solubility in stationary phase - Nature of mobile phase

#8
Front (Question)

Separation method of choice for amino acids, proteins, water and fat-soluble vitamins, antibiotics

Back (Answer)

HPLC

#9
Front (Question)

What are GC columns made of?

Back (Answer)

Glass or fused silica tubes

#10
Front (Question)

In GC, this phase is a liquid immobilised inside the column

Back (Answer)

stationary phase

#11
Front (Question)

This method can be used for ~85% of all organic compounds

Back (Answer)

HPLC

#12
Front (Question)

GC works for approx ____% of all organics

Back (Answer)

20%

#13
Front (Question)

2 benefits of using smaller, microbore columns in HPLC?

Back (Answer)

• Better resolution of peaks - Less solvent use

#14
Front (Question)

A compound that is chemically similar to the analyte, but can be detected separately

Back (Answer)

Internal standard

#15
Front (Question)

Elution method where there is a change in acetonitrile (mobile phase) throughout the experiment

Back (Answer)

Gradient elution

#16
Front (Question)

2 factors that affect how compounds are separated on a GC column?

Back (Answer)

• AFFINITY of the solute for the liquid - VOLATILITY of the solute

#17
Front (Question)

2 clinical applications of headspace sampling?

Back (Answer)

• Blood alcohol determination - Ethylene oxide residue in medical devices

#18
Front (Question)

Separation method of choice for detecting cocaine, heroin, ethanol, steroids, aromatics?

Back (Answer)

GC

#19
Front (Question)

This value helps eliminate possibilities, but cannot give a positive compound ID

Back (Answer)

Retention time (tr)

#20
Front (Question)

GC can only be used for __________ and ___________ compounds

Back (Answer)

volatile thermally-stable

#21
Front (Question)

In HPLC, which phase is polar? Nonpolar?

Back (Answer)

Polar - mobile Nonpolar - stationary

#22
Front (Question)

3 main components of a mass spectrometer?

Back (Answer)

• Ion source • Mass filter (analyser) • Detector

#23
Front (Question)

Multiple __________ are frequently used in order to generate calibration graphs for chromatography quantitative analysis

Back (Answer)

standards