Mass Spectrometry and Colorimetric / Spectrophotometric Techniques
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what is Mass Spectrometry
An analytical technique that ionizes chemical species and sorts the ions based on their mass-to-charge ratio.
how does Mass Spectrometry work
• sample ionized into charged particles. • Ions separated by their mass-to-charge ratio in a magnetic or electric field. • detector measures abundance of each ion
advantages of Mass Spectrometry
• sensitive and precise • can identify different compounds even with similar masses • provides molecular structure information
what are limitations of Mass Spectrometry
• expensive • requires specialized equip and training • sample prep can be complex
what are Colorimetric / Spectrophotometric Techniques
technique that measures the amount of light absorbed by a coloured solution at specific wavelengths
how do Colorimetric / Spectrophotometric Techniques work
• light beam passes through sample solution • light absorbed is measured • concentration calculated using the beer - lambert law
advantages to Colorimetric / Spectrophotometric Techniques
• quick and easy • suitable coloured solutions • non destructive to the sample
what are limitations of Colorimetric / Spectrophotometric Techniques
• interference from other coloured substances or impurities • requires calibration
what is Mass Spectrometry used for in the industy
detecting and identifying antibiotics, hormones and contaminates in animal products and the fermentation process
what is Colorimetric / Spectrophotometric Techniques used for in the industry
accurate testing for animal health, food safety and quality controls. helps prevent harmful contaminates and optimise beneficial microbial activity