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Module 3a: Photometry and Spectrophotometry

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Total cards: 24•Content version: v4•Public deck•Updated: 8/1/2026

Card preview (24)

#1
Front (Question)

majority of analytic techniques fall into one of four basic disciplines within the field of analytic chemistry which are

Back (Answer)

> spectrometry luminescence electroanalytic methods chromatography

#2
Front (Question)

five types of Spectrophotometry/Photometry/Colorimetry

Back (Answer)

a. Spectrophotometry b. Flame Emission Spectrophotometry c. Atomic Absorption Spectrophotometry d. Reflectance Spectrophotometry e. Mass Spectrophotometry

#3
Front (Question)

Light energy is a form of ________ radiation

Back (Answer)

Electromagnetic

#4
Front (Question)

light travels in packets of radiant energy called _____ or _____

Back (Answer)

photons or quanta

#5
Front (Question)

light energy is characterized by _______ and _______

Back (Answer)

frequency and wavelength

#6
Front (Question)

definition: FREQUENCY

Back (Answer)

number of vibrations of wave per second, has a direct relationship with energy

#7
Front (Question)

definition: WAVELENGTH

Back (Answer)

distance between two successive peaks in nanometers, has an inverse relationship with energy and frequency

#8
Front (Question)

light is used to describe radiant energy from the spectrum _____ to ____ nm

Back (Answer)

290 to 750 nm (ultraviolet to visible light)

#9
Front (Question)

differentiate: PHOTOMETRY and SPECTROPHOTOMETRY

Back (Answer)

both are measurements of the luminous intensity of light, but spectrophotometry measures only light at a selected wavelength

#10
Front (Question)

define: Planck’s theory

Back (Answer)

theory that energy is transmitted through units of quanta or photons where the more waves there are (higher frequency), the more light energy

#11
Front (Question)

basic components of a spectrophotometer according to the path of light

Back (Answer)

• Light source • Entrance slit • Monochromator • Exit slit • Cuvette with sample • Photodetectors • Meter or Readout devices

#12
Front (Question)

define: Beer’s Law

Back (Answer)

The concentration of a substance is directly proportional to the amount of light absorbed, or is inversely proportional to the log of the transmitted light.

#13
Front (Question)

Beer’s law formula

Back (Answer)

A = abc A (absorbance) a (proportionality constant defined as absorptivity) b (light path in cm) c (concentration in g/l)

#14
Front (Question)

light that has already passed through sample

Back (Answer)

transmitted light

#15
Front (Question)

light that hasn’t passed through a sample

Back (Answer)

incident light

#16
Front (Question)

percent transmittance is the ratio of ____

Back (Answer)

sample beam signal/ blank beam signal

#17
Front (Question)

% transmittance when all light is absorbed

Back (Answer)

0%

#18
Front (Question)

% transmittance when no light is absorbed

Back (Answer)

100%

#19
Front (Question)

used to measure the light transmitted by a solution to determine the concentration of the light-absorbing substance in the solution.

Back (Answer)

spectrophotometer

#20
Front (Question)

Most common light source in the visible portion of the spectrum, with most emitted in the infrared region.

Back (Answer)

Tungsten/Tungsten Iodide Lamp

#21
Front (Question)

Tungsten/Tungsten Iodide Lamp does not supply sufficient radiant energy or measurements below ____ nm

Back (Answer)

320 nm

#22
Front (Question)

Provide sources of continuous spectra in the UV region with some sharp emission lines.

Back (Answer)

Hydrogen and deuterium lamps

#23
Front (Question)

Deuterium lamps provide continuous emission down to ___ nm.

Back (Answer)

165 nm

#24
Front (Question)

Advantage of deuterium lamp over Hydrogen lamp

Back (Answer)

more stable and has longer life